4125 – AKLIDES® ASA

The AKLIDES® ASA is an immunofluorescence assay (IFA) for the qualitative determination of IgG antibodies in human serum against skin antigens on monkey esophagus sections. The AKLIDES® ASA is intended as an aid in the diagnosis of autoimmune skin diseases in conjunction with other clinical and laboratory findings.

Pemphigus, a group of chronic disorders, is often caused by immunological phenomena, which help to clinically differentiate them. Histological findings and the presence of characteristic autoantibodies further aid the differentiation. Indirect immuno­fluorescence tests on epithelial tissue sections, as well as immunohistological determination of skin biopsies are used for the detection of circulating antibodies, especially autoantibodies of IgG class, which are directed against intercellular epithelial antigens such as desmoglein III and I. Titers are found to increase in parallel with disease activity and decrease with successful therapy.

The IFA (indirect immunofluorescence assay) is an immunoassay for the determination of specific antibodies. Tissue sections or cells containing the antigen of interest are immobilized on slides. If specific antibodies are present in the patient´s sample, they bind to the antigen in the tissue. A secondary antibody conjugated with fluorescein-isothiocyanat (FITC) detects the generated immune complex. The slides are examined using a fluorescence microscope. A specific fluorescent staining pattern demonstrates the presence of specific antibodies in the patient´s sample.

The immunoassay is designed for manual processing and automated imaging using the AKLIDES® or akiron® system.

Worldwide first and fully automated system, AKLIDES® digital fluorescence microscope is used for the standardized imaging and data processing in immunofluorescence tests on glass slides. Controlled by the AKLIDES® Software, the AKLIDES® system has the ability to produce valid standardized analyses and to document immunofluorescence assays electronically to augment daily laboratory routine in the diagnosis of autoimmune diseases.

The immunoassay is designed for professional in vitro diagnostic use.

Product summary

Title AKLIDES®ASA
Product code 4125
Indication Autoimmune skin diseases
Description Indirect immunofluorescence assay for the determination of IgG antibodies in human serum against skin antigens
Format Slides coated with cryostat tissue sections of monkey esophagus
Incubation time 60 min.
Sample volume 10 µL serum
No. of determinations 48 (12 x 4)


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