4125 – AKLIDES® ASA
The AKLIDES® ASA is an immunofluorescence assay (IFA) for the qualitative determination of IgG antibodies in human serum against skin antigens on monkey esophagus sections. The AKLIDES® ASA is intended as an aid in the diagnosis of autoimmune skin diseases in conjunction with other clinical and laboratory findings. The immunoassay is designed for manual processing and automated imaging using the AKLIDES® or akiron® system. The immunoassay is designed for professional in vitro diagnostic use.
Pemphigus, a group of chronic disorders, is often caused by immunological phenomena, which help to clinically differentiate them. Histological findings and the presence of characteristic autoantibodies further aid the differentiation. Indirect immunofluorescence tests on epithelial tissue sections, as well as immunohistological determination of skin biopsies are used for the detection of circulating antibodies, especially autoantibodies of IgG class, which are directed against intercellular epithelial antigens such as desmoglein III and I. Titers are found to increase in parallel with disease activity and decrease with successful therapy.
|Indication||Autoimmune skin diseases|
|Description||Indirect immunofluorescence assay for the determination of IgG antibodies in human serum against skin antigens|
|Format||Slides coated with cryostat tissue sections of monkey esophagus|
|Incubation time||60 min.|
|Sample volume||10 µL serum|
|No. of determinations||48 (12 x 4)|