5014 – DotDiver CeliAK IgA

The DotDiver CeliAK IgA is a qualitative dot immunoassay for the determination of IgA antibodies against deamidated gliadin (d-gliadin) and tissue transglutaminase (tTG) in human serum. The DotDiver CeliAK IgA is intended as an aid in the diagnosis of celiac disease in conjunction with other clinical and laboratory findings.

Small intestinal damages leading to a so-called “flat” mucosa is characteristics of celiac disease or gluten-sensitivity. Due to these extensive lesions, malabsorption occurs frequently accompanied with a depletion of key nutrients. The diagnosis of celiac disease involves small intestine biopsy showing a “flat” mucosa prior to a gluten-free diet, which is then followed by reconstitution of the mucosa after onset of the diet.

The alcohol soluble fraction of gluten, gliadin, a substrate of tissue transglutaminase, which cross-links into high molecular complexes triggering probably both cellular and humoral immune responses, is the causative agent of celiac disease. Determination of IgG and IgA to deamidated gliadin as well as the detection of human endomysial autoantigens (EmA) IgA is considered as the main serological parameters for celiac disease so far.

Dot immunoassays are frequently used for the determination of specific antibodies directed against multiple antigens. The test strips are coated with various antigens in consistent intervals. If antibodies are present in the patient´s sample, they bind to the respective antigens. A secondary antibody conjugated with the enzyme alkaline phosphatase detects the generated immune complexes. A colorless substrate is converted into a colored, insoluble product. The signal intensity of the precipitated reaction product is proportional to the antibody activity in the sample.

The DotDiver CeliAK IgA is designed for semi-automated use with the DotDiver instrument.

The GA Generic Assays DotDiver2.0 is a fully automated processor of Line and Dot immunoassays. The small footprint renders the device an ideal solution for laboratories with small space. With a unique software design, the device provides a completely hands-free experience for quantitative, semi-quantitative and qualitative testing via repeated diving of the antigen coated strips into the ready to use cartridges. This special setup provides an optimal reaction environment with reduced assay processing and result analysis time for up to 24 different tests or samples simultaneously.