4291 – ANA 18 LINE
The ANA 18 LINE is a qualitative line immunoassay for the determination of IgG antibodies against nuclear and cytoplasmic antigens (dsDNA, nucleosome, Sm, Sm/RNP, ribosomes, histone, U1-snRNP, SS-A 60, SS-A 52, SS-B, Scl-70, PM/Scl, CENP-A/B, PCNA, Jo-1, M2, DFS-70 and f-Actin) in human serum. The ANA 18 LINE is intended as an aid in the diagnosis of systemic inflammatory rheumatic diseases in conjunction with other clinical and laboratory findings.
The detection of antibodies in patient samples is well established and plays an essential role in the diagnosis of systemic autoimmune diseases. The appearance of antibodies directed against components of the cell nucleus are characteristic features of systemic autoimmune diseases, especially systemic lupus erythematosus (SLE), Sjögren’s syndrome, progressive systemic sclerosis (PSS), mixed connective tissue disease (MCTD), rheumatoid arthritis (RA) and dermatomyositis.
Line immunoassays are frequently used for the determination of specific antibodies directed against multiple antigens. The test strips are coated with various antigens in consistent intervals. If antibodies are present in the patient´s sample, they bind to the respective antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complexes. A colorless substrate is converted into a colored, insoluble product. The signal intensity of the precipitated reaction product is proportional to the antibody activity in the sample.
The ANA 18 LINE is designed for manual professional in vitro diagnostic use.
|Title||ANA 18 LINE|
|Indication||Systemic inflammatory rheumatic diseases|
|Description||Line immunoassay for the qualitative determination of IgG antibodies against nuclear and cytoplasmic antigens in human serum|
|Format||Test strips coated with dsDNA, nucleosome, Sm, Sm/RNP, ribosomes, histone, U1-snRNP, SS-A 60, SS-A 52, SS-B, Scl-70, PM/Scl, CENP-A/B, PCNA, Jo-1, M2, DFS-70 and f-Actin|
|Total incubation time||80 min.|
|Sample volume||10 µL serum|
|No. of determinations||20 x 18|