4044 – Anti-hu tTG IgG

Highlights

• Use of IgG antibodies against tissue transglutaminase (tTG)
• Ready-to-use (exception: wash buffer), color- and barcoded reagents
• Quality assured handling in routine laboratories
• Short incubation times (60 min / 30 min / 15 min) at room temperature
• Quantitative determination of of IgG antibodies
• Detects tTG IgG autoantibodies, including in IgA-deficient celiac patients
• High precision and linearity within the measurement range
• CE marked
• Automatable

Intended Purpose

The Anti-hu tTG IgG is a quantitative immunoassay for the determination of IgG antibodies against tissue transglutaminase in human serum. The Anti-hu tTG IgG is intended as an aid in the diagnosis of celiac disease in conjunction with other clinical and laboratory findings. The immunoassay is designed for manual professional in vitro diagnostic use.

Diagnostic Relevance

Celiac disease or gluten-sensitivity is characterized by small intestinal damages leading to a so-called “flat” mucosa. Due to these extensive lesions, malabsorption occurs frequently accompanied with a depletion of key nutrients. The alcohol soluble fraction of gluten, gliadin, a substrate of tissue transglutaminase (tTG), represents a causative agent of celiac disease, by stimulating an inflammatory process in the small intestine. Cellular and humoral immune responses are probably triggered by the cross linking of gliadin into high molecular complexes.

Clinicians diagnose celiac disease by performing a small intestine biopsy that shows a “flat” mucosa before starting a gluten-free diet, followed by mucosal recovery after the diet begins. They primarily use ELISA to measure IgG and IgA antibodies to deamidated gliadin, along with detection of human endomysial autoantigen (EmA) IgA, as the main serological parameters for celiac disease.

Clinicians detect IgA and IgG antibodies to tTG and deamidated gliadin as part of celiac disease diagnosis. IgA antibodies to tTG provide the highest predictive value. However, IgA-deficient patients, a common occurrence in celiac disease, produce IgG autoantibodies only to tTG. In these patients, IgG autoantibodies to tTG, along with Gliadin IgG antibodies, serve as the primary serological markers for celiac disease.

Publications

• Grossmann et al., (2016) Simultaneous detection of celiac disease-specific IgA antibodies and total IgA
• Conrad et al., (2012) A new dot immunoassay for simultaneous detection of celiac specific antibodies and IgA-deficiency