4044 – Anti-hu tTG IgG
The Anti-hu tTG IgG is a quantitative immunoassay for the determination of IgG antibodies against tissue transglutaminase in human serum. The Anti-hu tTG IgG is intended as an aid in the diagnosis of celiac disease in conjunction with other clinical and laboratory findings.
Celiac disease or gluten-sensitivity is characterized by small intestinal damages leading to a so-called “flat” mucosa. Due to these extensive lesions, malabsorption occurs frequently accompanied with a depletion of key nutrients. The alcohol soluble fraction of gluten, gliadin, a substrate of tissue transglutaminase (tTG), represents a causative agent of celiac disease, by stimulating an inflammatory process in the small intestine. Cellular and humoral immune responses are probably triggered by the cross linking of gliadin into high molecular complexes.
The diagnosis of celiac disease involves small intestine biopsy showing a “flat” mucosa prior to a gluten-free diet, which is then followed by reconstitution of the mucosa after onset of the diet. Determination of IgG and IgA to deamidated gliadin by ELISA as well as the detection of Human endomysial autoantigens (EmA) IgA has been considered as the main serological parameters for celiac disease so far.
Serological method of celiac disease diagnosis includes the detection of IgA and IgG antibodies to tTG and deamidated gliadin. IgA antibodies to tTG are considered to have the highest predictive value, however, patients suffering from IgA deficiency, a frequent concomitant disease in celiac patients, demonstrate IgG autoantibodies to only tTG, hence apart from Gliadin IgG antibodies, IgG autoantibodies against tTG are the only serological parameter for the detection of celiac disease in such patients.
The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay for the determination of specific antibodies. The strips of the microtiter plate are coated with test-specific antigens. If antibodies are present in the patient´s sample, they bind to the antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. A colorless substrate is converted into the colored product. The signal intensity of the reaction product is proportional to the antibody activity in the sample. After stopping the signal intensity of the reaction product is measured photometrically.
The immunoassay is designed for manual professional in vitro diagnostic use.
|Title||Anti-hu tTG IgG|
|Description||Enzyme immunoassay for the quantitative determination of IgG antibodies against tissue transglutaminase in human serum|
|Format||Microtiter plate coated with recombinant human tissue transglutaminase|
|Total incubation time||105 min.|
|Sample volume||10 µL serum|
|No. of determinations||96 (89 x 1) + 5 x Calibrators + 2 x Controls|