4041 – Anti-ß2 GP-I
The Anti-ß2-GP-I is a quantitative immunoassay for the determination of IgG and / or IgM antibodies against b2-glycoprotein I (ß2-GP I) in human serum. The Anti-ß2-GP-I is intended as an aid in the diagnosis of anti-phospholipid antibody syndrome (APAS) in conjunction with other clinical and laboratory findings. The immunoassay is designed for manual professional in vitro diagnostic use.
Anti-phospholipid antibody syndrome (APAS), an autoimmune disorder evident by clinical symptoms such as thrombocytopenia, arterial (venous) thrombosis, and recurrent foetal loss, as well as systemic lupus erythematosus (SLE) are characterized by the presence of autoantibodies reactive to negatively charged phospholipids. The detection of such autoantibodies is well established and plays an essential role in the diagnostics of systemic autoimmune diseases.
In autoimmune patients, phospholipid antibodies seem to recognize phospholipids associated with plasma protein cofactors, e.g. b2 glycoprotein-I (ß2 GP-I), a serum protein with a molecular weight of ca. 50 kDa, that affects platelet aggregation and coagulation. Negatively charged phospholipids such as Cardiolipin interact with the positively charged fifth domain of ß2 GP-I, an interaction that leads to conformational changes of the protein and the creation of new epitopes recognized by autoimmune phospholipid autoantibodies.
|Indication||Anti-phospholipid antibody syndrome (APAS)|
|Description||Enzyme immunoassay for the determination of IgG and / or IgM antibodies against ß2-glycoprotein I (ß2-GP I) in human serum|
|Format||Microtiter plate coated with ß2-glycoprotein I|
|Total incubation time||105 min.|
|Sample volume||10 µL serum|
|No. of determinations||96 (89 x 1) + 5 x Calibrators + 2 x Controls|