86448 – Anti-GBM IFA

Intended Purpose

The Anti-GBM IFA is an immunofluorescence assay (IFA) for the qualitative and semi-quantitative determination of IgG antibodies in human serum against glomerular basement membrane (GBM) on tissue sections of monkey kidney. The Anti-GBM IFA is intended as an aid in the diagnosis of autoimmune glomerulonephritides in conjunction with other clinical and laboratory findings. The immunoassay is designed for manual professional in vitro diagnostic use.

Diagnostic Relevance

Goodpasture syndrome, an autoimmune kidney disorder, is characterized by the coexistence of proliferative glomerulonephritis with lung haemorrhage and the formation of anti-GBM antibodies. Detection of the pathogenic circulating autoantibodies to GBM is a key diagnostic parameter of Goodpasture syndrome. The GBM is an anatomical barrier between kidney epithelia and connective tissue and plays an important role in ultrafiltration. Rapid progressive glomerulonephritis (RPGN) is a common feature of many autoimmune disorders. Differential diagnosis of autoimmune nephritides requires the determination of antibodies to GBM together with the determination of antibodies to neutrophilic cytoplasmic antigens (ANCA, characteristic for Wegener’s disease and vasculitis-associated RPGN), and nuclear antibodies (ANA, characteristic for lupus nephritis).