86048, 86096 – EmA IFA

Highlights

• Slides coated with monkey esophagus tissue sections
• Specific detection of IgA antibodies against endomysium
• Supports the diagnosis of celiac disease
• Quality assured handling in routine laboratories
• Consistent processing for parallel use of multiple IF assays
• High diagnostic sensitivity and specificity
• Designed for manual professional in vitro diagnostic use
• CE marked

Intended Purpose

The EmA IFA is an immunofluorescence assay (IFA) for the qualitative and semi-quantitative determination of IgA antibodies in human serum against endomysium on tissue sections of monkey esophagus. The EmA IFA is intended as an aid in the diagnosis of celiac disease conjunction with other clinical and laboratory findings. The immunoassay is designed for manual professional in vitro diagnostic use only.

Diagnostic Relevance

Celiac disease or gluten-sensitivity is characterized by small intestinal damages leading to a so-called “flat” mucosa. Due to this extensive lesions, malabsorption occurs frequently accompanied with a depletion of key nutrients. Damage of the enterocytic membrane, including decrease in activity of the brush border enzyme leads to the activation of the local immune response leading to villious atrophy. Dermatitis herpetiformis, an autoimmune chronic relapsing skin disease, demonstrates celiac-like changes in the intestine, resembling the inflammatory process in celiac patients. Sub-epidermal blisters form in this disease, with IgA antibodies in the basal membrane and infiltration of neutrophils and eosinophils.

Publications

• Conrad et al., (2012) A new dot immunoassay for simultaneous detection of celiac specific antibodies and IgA-deficiency