84048 – ASMA IFA

Highlights

• Slides coated with rat stomach tissue sections for specific detection of IgG antibodies against smooth muscle
• Detects anti-smooth muscle antibodies (ASMA) as markers for autoimmune liver diseases
• Supports diagnosis of acute and chronic hepatitis, primary biliary cirrhosis, liver cirrhosis, SLE, and other systemic conditions
• Clear fluorescence patterns in muscularis mucosa and tunica muscularis allow precise identification
• Includes ready-to-use substrate slides, positive and negative controls, conjugates, PBS buffer, mounting medium, blotting templates, and coverslips
• Manual procedure optimized for professional in vitro diagnostic use
• CE marked for in vitro diagnostic applications

Intended Purpose

The ASMA IFA is an immunofluorescence assay (IFA) for the qualitative and semi-quantitative determination of IgG antibodies in human serum against smooth muscle on tissue sections of rat stomach. The ASMA IFA is intended as an aid in the diagnosis of autoimmune liver diseases in conjunction with other clinical and laboratory findings. The immunoassay is designed for manual professional in vitro diagnostic use.

Diagnostic Relevance

Autoimmune diseases result from a disruption of normal cellular or humoral immune reactions, where the immune system mistakenly targets the body’s own cells, leading to tissue damage and disease. In the liver, this can manifest as acute or chronic hepatitis, primary biliary cirrhosis, and other forms of liver cirrhosis. These conditions are often associated with antibodies against smooth, unstriated muscle fibers, which serve as key diagnostic markers known as anti-smooth muscle antibodies (ASMA).

Beyond liver-specific autoimmune conditions, ASMA detection can support the diagnosis of systemic diseases such as systemic lupus erythematosus (SLE), infectious mononucleosis, and certain malignancies, including breast and ovarian carcinoma as well as malignant melanomas.

The ASMA IFA employs rat stomach tissue sections, where positive results produce distinct fluorescence patterns. Smooth muscle fibers in the muscularis mucosa and tunica muscularis ventriculi exhibit bright staining, while inter-glandular contractile fibrils of the gastric mucosa also fluoresce in the presence of ASMA. This clear, specific staining enables reliable identification of antibodies, helping clinicians confirm autoimmune liver involvement, differentiate it from other conditions, and monitor disease progression or therapeutic response.