4220 – BiermAK LINE

The BiermAK LINE is a qualitative line immunoassay for the determination of IgG antibodies against intrinsic factor and parietal cell antigens in human serum. The BiermAK LINE is intended as an aid in the diagnosis of chronic atrophic gastritis in conjunction with other clinical and laboratory findings.

The main antigen of autoantibodies to parietal cells is the membrane H+/K+ ATPase transporting H+ into the stomach. Antibodies to this ATPase are a sensitive marker for type A gastritis and will be found in 80-90% of such patients. Autoantibodies to intrinsic factor are detected mainly in patients with pernicious anemia associated with gastritis and are pathognomonic for this clinical picture.

Line immunoassays are frequently used for the determination of specific antibodies directed against multiple antigens. The test strips are coated with various antigens in consistent intervals. If antibodies are present in the patient´s sample, they bind to the respective antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complexes. A colorless substrate is converted into a colored, insoluble product. The signal intensity of the precipitated reaction product is proportional to the antibody activity in the sample.

The BiermAK LINE is designed for manual professional in vitro diagnostic use.

 

Product summary

Title BiermAK LINE
Product code 4220
Indication Chronic atrophic gastritis
Description Line immunoassay for the qualitative determination of IgG antibodies against intrinsic factor and parietal cell antigens in human serum
Format Test strips coated with intrinsic factor and H+/K+ ATPase
Total incubation time 80 min.
Sample volume 20 µL serum
No. of determinations 20 x 2


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