4058 – Anti-MPO

The Anti-MPO is a quantitative immunoassay for the determination of IgG antibodies against myeloperoxidase (MPO) in human serum. The Anti-MPO is intended as an aid in the diagnosis of systemic vasculitides (SV) in conjunction with other clinical and laboratory findings.

Systemic vasculitis (SV) pathogenesis is majorly identified by the inflammation of different blood vessel walls, and the resulting morphological changes. Arteries and veins can both be affected simultaneously. The clinical manifestation is mainly indicated by general symptoms like exhaustion, fever and weight loss. Depending on which kind of vessels are affected, future disease course varies.

Anti-neutrophil cytoplasmic antibodies (ANCA) play an important role in the serological diagnosis of SV. These antibodies are usually determined by indirect immunofluorescence (IIF) of ethanol-fixed human neutrophils, in which the pattern of the IIF distinguishes cytoplasmic ANCA (cANCA) and perinuclear ANCA (pANCA). In IIF, the pattern of pANCA is mainly caused by the reactivity of MPO, a cationic protein found in azurophilic granules. MPO autoantibodies occur in a variety of vasculitides such as microscopic polyangiitis, Churg-Strauss syndrome and Polyarteritis nodosa. Autoantibodies against MPO are correlated with idiopathic or vasculitis associated necrotizing crescentic glomerulonephritis and are found frequently in 70% of patients with microscopic polyangilitis, and 5-50% of patients with Churg-Strauss syndrome.

The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay for the determination of specific antibodies. The strips of the microtiter plate are coated with test-specific antigens. If antibodies are present in the patient´s sample, they bind to the antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. A colorless substrate is converted into the colored product. The signal intensity of the reaction product is proportional to the antibody activity in the sample. After stopping the signal intensity of the reaction product is measured photometrically.

The immunoassay is designed for manual professional in vitro diagnostic use.

Product summary

Title Anti-MPO
Product code 4058
Indication Systemic vasculitis
Description Enzyme immunoassay for the quantitative determination of IgG antibodies against myeloperoxidase (MPO) in human serum
Format Microtiter plate coated with purified human myeloperoxidase (MPO) from neutrophil granulocytes
Total incubation time 105 min.
Sample volume 10 µL serum
No. of determinations 96 (89 x 1) + 5 x Calibrators + 2 x Controls

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