4049 – PmSclplus Dot

The PmSclplus Dot is a qualitative dot immunoassay for the determination of IgG antibodies against nuclear and cytoplasmic antigens (Jo-1, PL-7, PL-12, SRP, Mi-2, Ku, PM-Scl 100 and Scl-70) in human serum. The PmSclplus is intended as an aid in the diagnosis of systemic autoimmune diseases in conjunction with other clinical and laboratory findings.

Progressive systemic sclerosis (PSS) or systemic scleroderma is an autoimmune disease of connective tissue characterized by slowly progressive fibrosis leading to sclerosis. Scl-70 autoantibodies are pathognomonic for PSS patients.

Polymyositis and dermatomyositis are idiopathic inflammatory muscle disorders with an unknown etiology, however, autoantibodies to t-RNA transferases like Jo-1, PL-7, and PL-12 are found in myositis patients. Antibodies to SRP are also specific markers for Polymyositis. Mi-2 autoantibodies are detected almost exclusively in patients with dermatomyositis. Patients tested positive for Mi-2 have a relatively mild clinical course.

Dot immunoassays are frequently used for the determination of specific antibodies directed against multiple antigens. The test strips are coated with various antigens in consistent intervals. If antibodies are present in the patient´s sample, they bind to the respective antigens. A secondary antibody conjugated with the enzyme alkaline phosphatase detects the generated immune complexes. A colorless substrate is converted into a colored, insoluble product. The signal intensity of the precipitated reaction product is proportional to the antibody activity in the sample.

The PmSclplus Dot is designed for manual professional in vitro diagnostic use.

Product summary

Title PmSclplus Dot
Product code 4049
Indication Systemic autoimmune diseases
Description Dot Immunoassay for the qualiative determination of IgG antibodies against nuclear and cytoplasmic antigens in human serum
Format Test strips coated with Jo-1, PL-7, PL-12, SRP, Mi-2, Ku, PM-Scl 100 and Scl-70
Total incubation time 80 min.
Sample volume 10 µL serum
No. of determinations 24 x 8

Restricted downloads - Password required