3002 – Medizym® anti-TPO
The Medizym® anti-TPO is a quantitative immunoassay for the determination of IgG antibodies against thyroid peroxidase (TPO) in human serum. The Medizym® anti-TPO is intended as an aid in the diagnosis of autoimmune diseases of thyroid gland in conjunction with other clinical and laboratory findings.
Thyroid peroxidase (TPO), a membrane-bound enzyme in the thyroid cell, plays an essential role in the synthesis of thyroid hormone, hence makes up one of the three thyroid antigens. The presence of autoantibodies to thyroid antigens is a major indication of autoimmune thyroiditis. These autoantibodies target the thyroid glands and its hormone products, thyroxine (T4), triiodothyronine (T3) and thyroid stimulating hormone (TSH), leading to hormonal dysfunction. Detection of such autoantibodies is an indication of chronic autoimmune thyroiditis, hence a marker for the differential diagnosis of hypothyroidism and the subclinical or latent type. Detection of anti-TPO is also essential in the diagnosis of Grave’s disease, especially when the TSH Receptor Antibody value is negative, but the disease is clinically suspected. Besides Grave’s diseases, anti-TPO determination is also an indication of the exclusion of a co-existing thyroid autoimmune disease, as in euthyroid goiter.
The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay for the determination of specific antibodies. The strips of the microtiter plate are coated with test-specific antigens. If antibodies are present in the patient´s sample, they bind to the antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. A colorless substrate is converted into the colored product. The signal intensity of the reaction product is proportional to the antibody activity in the sample. After stopping the signal intensity of the reaction product is measured photometrically.
The immunoassay is designed for manual professional in vitro diagnostic use.
|Description||Enzyme immunoassay for the quantitative determination of IgG antibodies against thyroid peroxidase (TPO) in human serum|
|Format||Microtiter plate coated with recombinant human TPO|
|Total incubation time||105 min.|
|Sample volume||10 µL serum|
|No. of determinations||96 (90 x 1) + 4 x Calibrators + 2 x Controls|