4271 – AKLIDES^® CytoBead^® CeliAK

Highlights

• Slides coated with cryostat tissue sections of monkey esophagus and beads coated with tTG, deamidated gliadin (DG), and anti-human IgA
• Sensitive detection of IgA or IgG antibodies for celiac disease diagnosis
• Ready-to-use reagents (exception: PBS buffer)
• Quality assured handling in routine laboratories
• Short incubation times (30 min / 30 min) at room temperature
• Consistent processing for parallel use of multiple IF assays
• High diagnostic sensitivity and specificity
• Automated imaging by use of AKLIDES® or akiron® systems
• CE marked

Intended Purpose

The AKLIDES^® CytoBead^® CeliAK is an immunofluorescence assay (IFA) for the qualitative determination of IgA antibodies in human serum against endomysium (EmA) as well as for the quantitative determination of IgA antibodies against tissue transglutaminase (tTG) and deamidated gliadin (dG) as well as for control of IgA antibodies. The IgG conjugate allows for the qualitative determination of IgG antibodies against endomyium, tTG and dG in case of IgA deficiency. The AKLIDES® CytoBead® CeliAK helps clinicians diagnose celiac disease alongside other clinical and laboratory findings. Laboratory staff perform the assay manually, and the AKLIDES® or akiron® systems handle automated imaging. The immunoassay is designed for professional in vitro diagnostic use only.

Diagnostic Relevance

Celiac disease (CD) or gluten-sensitivity is characterized by small intestinal damages leading to a so-called “flat” mucosa. Due to this extensive lesions, malabsorption occurs frequently accompanied with a depletion of key nutrients. Highly specific autoantibodies target the common CD autoantigen tTG, along with antibodies against DG. EmA target extracellular tTG Except for DG antibodies these antibodies are typically of the IgA class. In IgA deficient patients with celiac disease, the same type of antibodies in IgG class can be detected.

Publications

• Abdukhakimovaova et al., (2021) Reliability of the Multiplex CytoBead CeliAK Immunoassay to Assess Anti-tTG IgA for Celiac Disease Screening
• Sowa et al., (2017) Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology
• Grossmann et al., (2016) Simultaneous detection of celiac disease-specific IgA antibodies and total IgA
• Sowa et al., (2014) Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen AutoAbsanalytik bei systemischen Autoimmunerkrankungen