4327 – AKLIDES® Cell Damage
AKLIDES Cell Damage system offers a standardized and automatic means of measuring and evaluating biomarkers for the determination of DNA double strand breaks, DNA repair foci. The quantitative determination of DNA repair foci via immunofluorescence is a very sensitive method, which is able to evaluate both single cells and single foci.
DNA damage such as DNA double-strand breaks (DSBs) can be measured following exposure to ionizing radiation or mutagenic substances such as cytostatic chemicals. Cells are usually able to identify and accurately repair this damage without any consequences to the cell. However, excessive DNA damage can lead to cell death or even oncogenic transformation of the cell. The knowledge of the formation and of the repair of DNA damage in particular are important requirements to avoid pathological changes and to ensure optimal application of ionizing radiation in medical technology.
The use of non-standardized protocols for sample preparation, and the subsequent manual evaluation of DNA repair foci, means that test results from different studies are not comparable. Using automatic analysis with the AKLIDES® Cell Damage system removes the subjective factor of visual counting, and so for the first time it is possible to compare test results from studies worldwide.
|Title||AKLIDES® Cell Damage assay|
|Size/Weight||65 cm x 67 cm x 32 cm (L x W x H) / 78 kg|
|Indication||DNA double strand breaks | Oncology | Stress study|
|Description||Standardized and automatic determination of DNA double strand breaks using immunofluorescence|
|Illumination||4 wavelengths LED|
|Objectives||4 different objectives (4 x, 10x, 40x, 60x)|
|Slide amount||5 slides|
|Analysis||Focus point analysis (ɣ-H2AX and 53BP1) in DAPI stained cells|
|Parameters||ɣ-H2AX and 53BP1|
|Report||Overall report & single report, Documentation, Archival storage|
|Connectivity||Pipettor (AKENOMI Neo), LIMS, Aklides System Software (Reading and evaluation)|