50381 – DotDiver Anti-Gangliosid IgG

The DotDiver Anti-Gangliosid IgG is a qualitative line immunoassay for the determination of IgG antibodies against gangliosides in human serum. The DotDiver Anti-Gangliosid IgG is intended as an aid in the diagnosis of autoimmune neuropathies in conjunction with other clinical and laboratory findings.

Clinical symptoms associated with inflammatory neuropathies of the peripheral nervous system range from mild signs like slight weariness, inexplicable indisposition to complicated cases such as functional deficiency like respiratory paralysis, cardiac arrest and neuromuscular disorders. Gangliosides, acid glycolipids containing a lipid (ceramide), oligosaccharide and sialic acid, are major components of cell membranes and found especially in the central and peripheral nervous system. Autoantibodies to gangliosides are detected in patients suffering from disorders of the peripheral nervous system.

Infection with Campylobacter jejuni, Cytomegalovirus, Epstein-Barr virus, Mycoplasma pneumoniae or Haemophilus influenza are risk factors for inflammatory neuropathies: Antibodies to ganglioside structures of these microorganisms may cross-react with gangliosides of the myelin sheath or neurofibre, leading to activation of inflammation processes with subsequent demyelination.

Line immunoassays are frequently used for the determination of specific antibodies directed against multiple antigens. The test strips are coated with various antigens in consistent intervals. If antibodies are present in the patient´s sample, they bind to the respective antigens. A secondary antibody conjugated with the enzyme horseradish peroxidase detects the generated immune complexes. A colorless substrate is converted into a colored, insoluble product. The signal intensity of the precipitated reaction product is proportional to the antibody activity in the sample.

The DotDiver Anti-Gangliosid IgG is designed for semi-automated use with the DotDiver instrument.

The GA Generic Assays DotDiver2.0 is a fully automated processor of Line and Dot immunoassays. The small footprint renders the device an ideal solution for laboratories with small space. With a unique software design, the device provides a completely hands-free experience for quantitative, semi-quantitative and qualitative testing via repeated diving of the antigen coated strips into the ready to use cartridges. This special setup provides an optimal reaction environment with reduced assay processing and result analysis time for up to 24 different tests or samples simultaneously.

Product summary

Title DotDiver Anti-Gangliosid IgG
Product code 50381
Indication Autoimmune neuropathies
Description Line immunoassay for the qualitative determination of IgG antibodies against gangliosides in human serum
Format Test strips coated with sulfatides, GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b and GQ1b
Total incubation time 95 min.
Sample volume 10 µL serum
No. of determinations 24 x 12

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