4208 – CeliAK IgA LINE
The CeliAK IgA LINE is a qualitative line immunoassay for the determination of IgA antibodies against deamidated gliadin (d-gliadin) and tissue transglutaminase (tTG) in human serum. The CeliAK IgA LINE is intended as an aid in the diagnosis of celiac disease in conjunction with other clinical and laboratory findings.
Celiac disease or gluten-sensitivity is characterized by small intestinal damages leading to a so-called “flat” mucosa. Due to this extensive lesions, malabsorption occurs frequently accompanied with a depletion of key nutrients. The alcohol soluble fraction of gluten, gliadin, a substrate of tissue transglutaminase (tTG), represents a causative agent of celiac disease, by stimulating an inflammatory process in the small intestine.
Serological method of celiac disease diagnosis includes the detection of IgA and IgG antibodies to tissue transglutaminase and deamidated gliadin. IgA antibodies to tissue transglutaminase are considered to have the highest predictive value, thus patients suffering from IgA deficiency, a frequent concomitant disease in celiac patients, can be diagnosed using IgG type antibodies.
Line immunoassays are frequently used for the determination of specific antibodies directed against multiple antigens. The test strips are coated with various antigens in consistent intervals. If antibodies are present in the patient´s sample, they bind to the respective antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complexes. A colorless substrate is converted into a colored, insoluble product. The signal intensity of the precipitated reaction product is proportional to the antibody activity in the sample.
The CeliAK IgA LINE is designed for manual professional in vitro diagnostic use.
|Title||CeliAK IgA LINE|
|Description||Line immunoassay for the qualitative determination of IgA antibodies against deamidated gliadin and tissue transglutaminase in human serum|
|Format||Test strips coated with highly purified deamidated gliadin and tissue transglutaminase|
|Total incubation time||80 min.|
|No. of determinations||20 x 2|