4067 – Anti-Faktor H

The Anti-Faktor H is a quantitative immunoassay for the determination of IgG antibodies against complement factor H (CFH) in human serum. The Anti-Faktor H is intended as an aid in the diagnosis of atypical hemolytic-uremic syndrome (HUS) in conjunction with other clinical and laboratory findings.

HUS is a disease of small blood vessels, characterized by hemolytic anemia, thrombocytopenia and acute renal failure, with toxin forming Escherichia coli bacteria (Shiga toxin, Vero toxin) being the most common cause is an infection with. This condition is due to disorder of complement regulation, a genetic mutations or antibodies to complement factor H.

The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay for the determination of specific antibodies. The strips of the microtiter plate are coated with test-specific antigens. If antibodies are present in the patient´s sample, they bind to the antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. A colorless substrate is converted into the colored product. The signal intensity of the reaction product is proportional to the antibody activity in the sample. After stopping the signal intensity of the reaction product is measured photometrically.

The immunoassay is designed for manual professional in vitro diagnostic use.