3900 – Anti-ASGPR

The Anti-ASGPR is a semi-quantitative immunoassay for the determination of IgG antibodies against asialoglycoprotein receptor (ASGPR) in human serum. The Anti-ASGPR is intended as an aid in the diagnosis of systemic liver autoimmune disorders in conjunction with other clinical and laboratory findings.

The level of ASGPR, a liver specific membrane receptor playing a pivotal role in the endocytosis of glycoproteins from the blood, correlates with the severity of inflammatory liver disorders especially autoimmune hepatitis, and declines under therapy. Patients suffering from autoimmune hepatitis (AIH), one of the primary autoimmune liver disease, including primary biliary cirrhosis and primary sclerosing cholangitis, show a variety of autoantibodies. Determination of autoantibodies to ASGPR is used in the follow-up and differential diagnosis of toxic and other chronic inflammatory liver disorders, in that autoantibodies to this parameter, ASGPR, can be detected in sera of up to 76% of patients suffering from AIH.

The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay for the determination of specific antibodies. The strips of the microtiter plate are coated with test-specific antigens. If antibodies are present in the patient´s sample, they bind to the antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. A colorless substrate is converted into the colored product. The signal intensity of the reaction product is proportional to the antibody activity in the sample. After stopping the signal intensity of the reaction product is measured photometrically.

The immunoassay is designed for manual professional in vitro diagnostic use.

Product summary

Title Anti-ASGPR
Product code 3900
Indication Autoimmune hepatitis
Description Enzyme immunoassay for the semi-quantitative determination of IgG antibodies against asialoglycoprotein receptor in human serum
Format Microtiter plate coated with purified ASGPR
Total incubation time 105 min.
Sample volume 10 µL serum
No. of determinations 96 (93 x 1) + 1 x Calibrator + 2 x Controls

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