1450 – SELco® TSH rapid
The SELco® TSH rapid is a quantitative immunoassay for the determination of thyrotropin (TSH) in human serum. The SELco® TSH rapid is intended as an aid in the diagnosis of thyroid diseases in conjunction with other clinical and laboratory findings.
The biosynthesis of Tg is regulated by thyroid-stimulating hormone (TSH), also known as thyrotropin, a pituitary hormone that stimulates the thyroid gland to produce thyroid hormones thyroxine (T4), and then triiodothyronine (T3). T4 and T3 regulate the secretion of TSH by a negative feedback mechanism; T3 or T4 elevation suppresses TSH in the serum, whereas their fall increase the level of TSH in serum, an early and specific indicator of primary hypothyroidism.
The presence of autoantibodies to thyroid antigens is an indication of autoimmune thyroiditis. These autoantibodies target the thyroid glands and its hormone products T3, T4 and TSH leading to hormonal dysfunction. Detection of such autoantibodies in an indication of chronic autoimmune thyroiditis, hence a marker for the differential diagnosis of hypothyroidism and the subclinical or latent type.
Radioimmunoassays based on coated tube technology are widely used for the determination of specific antigens. The SELco® TSH rapid immunoassay is based on a reaction of two monoclonal antibodies against thyrotropin (TSH), one biotinylated and another conjugated with Iodine-125. If thyrotropin is present in the patient´s sample, the antibodies bind simultaneously to different epitopes of the antigen. The generated immune complex is detected by streptavidin coated on the inner surface of the tubes. Decantation of the reaction mixture allows for the simple separation of generated immune complexes on the surface of the tube from the reaction mixture. The radioactivity of the tube is measured and proportional to the thyrotropin concentration in the patient´s sample.
The immunoassay is designed for manual professional in vitro diagnostic use.
|Title||SELco® TSH rapid|
|Description||Radioimmunoassay for the quantitative determination of Thyrotropin (TSH) in human serum|
|Format||Coated tube technology|
|Total incubation time||30 minutes|
|Sample volume||100 µL|
|No. of determinations||100 (91 x 1) + 7 x Calibrators + 2 x Controls|