4156 – AKLIDES® AAA

The AKLIDES® AAA is an immunofluorescence assay (IFA) for the qualitative determination of IgG antibodies in human serum against the adrenal cortex (AAA) on monkey adrenal gland sections. The AKLIDES® AAA is intended as an aid in the diagnosis of Addison’s disease in conjunction with other clinical and laboratory findings.

Adrenal antibodies are associated with the idiopathic form of Addison’s disease, and are more common in males than females. Many patients with adrenal antibodies also have an overlap of additional diseases such as thyroid disease, insulin-dependent diabetes, and secondary amenorrhoea. Screening for adrenal antibodies in these circumstances could be beneficial as very low incidence of adrenal antibody are found in normal cases. The anti-adrenal autoantibodies are organ specific antibodies only reacting with antigens found in adrenal gland. Non-organ specific antibodies like mitochondrial antibodies react with the adrenal cortex cells resembling AAA specific fluorescence.

The IFA (indirect immunofluorescence assay) is an immunoassay for the determination of specific antibodies. Tissue sections or cells containing the antigen of interest are immobilized on slides. If specific antibodies are present in the patient´s sample, they bind to the antigen in the tissue. A secondary antibody conjugated with fluorescein-isothiocyanat (FITC) detects the generated immune complex. The slides are examined using a fluorescence microscope. A specific fluorescent staining pattern demonstrates the presence of specific antibodies in the patient´s sample.

The immunoassay is designed for manual processing and automated imaging using the AKLIDES® or akiron® system.

Worldwide first and fully automated system,  AKLIDES® digital fluorescence microscope is used for the standardized imaging and data processing in immunofluorescence tests on glass slides for the automated determination of ANA, ANCA, nDNA, analysis of microparticles (Cytobead®) and for documentation of tissue sections. Controlled by the AKLIDES® Software, the AKLIDES® system has the ability to produce valid standardized analyses and to document immunofluorescence assays electronically to augment daily laboratory routine in the diagnosis of autoimmune diseases.

 The immunoassay is designed for professional in vitro diagnostic use.

Product summary

Title AKLIDES® AAA
Product code 4156
Indication Addison’s disease
Description Indirect IFA for the determination of antibodies against adrenal cortex antigens in human serum
Format Slides with monkey adrenal cortex
Incubation time 60 minutes
Sample volume 50 µl serum
No. of determinations 48 (12x4)


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