4133 – AKLIDES® CMA

The AKLIDES® CMA is an immunofluorescence assay (IFA) for the qualitative determination of IgG antibodies in human serum against cardiac muscle antigens (CMA) on monkey heart muscle sections. The AKLIDES® CMA is intended as an in the diagnosis of autoimmune cardiac diseases in conjunction with other clinical and laboratory findings.

Serologic assessment of cardiac diseases may be facilitated by the detection of CMA using indirect fluorescent antibody method. Identification of histologically defined circulating antibody to one or more of the cardiac muscle antigens can serve as diagnostic and prognostic marker for diseases like rheumatic fever, myocardial infarction and a variety of post-cardiotomy states.

The IFA (indirect immunofluorescence assay) is an immunoassay for the determination of specific antibodies. Tissue sections or cells containing the antigen of interest are immobilized on slides. If specific antibodies are present in the patient´s sample, they bind to the antigen in the tissue. A secondary antibody conjugated with fluorescein-isothiocyanat (FITC) detects the generated immune complex. The slides are examined using a fluorescence microscope. A specific fluorescent staining pattern demonstrates the presence of specific antibodies in the patient´s sample.

The immunoassay is designed for manual processing and automated imaging using the AKLIDES® or akiron® system.

Worldwide first and fully automated system, AKLIDES® digital fluorescence microscope is used for the standardized imaging and data processing in immunofluorescence tests on glass slides. Controlled by the AKLIDES® Software, the AKLIDES® system has the ability to produce valid standardized analyses and to document immunofluorescence assays electronically to augment daily laboratory routine in the diagnosis of autoimmune diseases.

The immunoassay is designed for professional in vitro diagnostic use.

Product summary

Title AKLIDES® CMA
Product code 4133
Indication Cardiac disease
Description Indirect IFA for the determination of antibodies against cardiac muscle antigens (CMA) in human serum
Format Slides with monkey heart muscle
Incubation time 60 minutes
Sample volume 10 µl serum
No. of determinations 48 (12x4)


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