Thyroid autoantibodies
Radioreceptor assay for the determination of TSH receptor autoantibodies (TRAb) in human serum
| Assay principle | Radio receptor assay (comp.) |
|---|---|
| Tracer | 125-Jod-anti-TSHR (mouse) |
| Standard range | 1 - 50 IU/l (WHO 08/204) |
| Cut off | 1.5 IU/l |
| Reaction type | solid phase (coated tubes with human TSH receptor) |
| Total incubation time | 180 minutes |
| Sample volume | 100 µl native serum |
| Functional assay sensitivity | 1 IU/l |
| Available format | 100 determinations |
Radioreceptor assay for the determination of TSH receptor autoantibodies (TRAb) in human serum
| Assay principle | Radio receptor assay (comp.) |
|---|---|
| Tracer | 125-Jod-anti-TSHR (mouse) |
| Standard range | 1 - 50 IU/l (WHO 08/204) |
| Cut off | 1.5 IU/l |
| Reaction type | solid phase (coated tubes with human TSH receptor) |
| Total incubation time | 120 min |
| Sample volume | 100 µl native serum |
| Functional assay sensitivity | 0.9 IU/l |
| Available format | 100 determinations |
Radioimmunoassay for the determination of autoantibodies to Thyroid Peroxidase (TPO Ab) in human serum
| Assay principle | RIA (competitive) |
|---|---|
| Tracer | 125-Iod-TPO (human) |
| Standard range | 0 - 1900 IU/ml |
| Cut off | 50 U/ml |
| Reaction type | solid phase (coated tubes with polyclonal anti-TPO) |
| Total incubation time | 120 minutes |
| Sample volume | 10 µl |
| Functional assay sensitivity | 12 IU/ml |
| Available format | 100 determinations |
Radioimmunoassay for the determination of autoantibodies to Thyroblobulin (Tg Ab) in neat serum. no predilution required
| Assay principle | RIA (competitive) |
|---|---|
| Tracer | 125-iod-Tg (human) |
| Standard range | 0 - 3000 IU/ml (NIBS 65/93) |
| Cut off | 100 IU/ml |
| Reaction type | solid phase (coated tubes) |
| Total incubation time | 120 minutes |
| Sample volume | 100 µl native serum |
| Functional assay sensitivity | 12 IU/ml |
| Available format | 100 determinations |
Thyroid hormone
Immunoradiometric assay for the determination of Thyrotropin (TSH) in serum
| Assay principle | IRMA |
|---|---|
| Tracer | 125-Iod-anti-hTSH mAb (mouse) |
| Standard range | 0.06 - 100 mIU/l (IRP 80/558) |
| Reaction type | solid phase (coated tubes) |
| Total incubation time | 30 minutes |
| Sample volume | 100 µl |
| Functional assay sensitivity | 0.03 mIU/l |
| Available format | 100 determinations |
Radioimmuno assay for the determination of free Triiodothyronine (FT3) in human serum
| Assay principle | RIA (one step) |
|---|---|
| Tracer | 125-iod-anti-T3 (monoclonal) |
| Standard range | 0 - 40 pmol/l |
| Reaction type | solid phase (coated tubes) |
| Total incubation time | 60 minutes |
| Sample volume | 100 µl serum |
| Analytical assay sensitivity | 0.3 pmol |
| Available format | 100 determinations |
Radioimmuno assay for the determination of free Thyroxine (FT4) in human serum
| Assay principle | RIA (one step) |
|---|---|
| Tracer | 125-Iod-anti-T4 (monoclonal) |
| Standard range | 0 - 80 pmol/l |
| Reaction type | solid phase (coated tubes) |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl serum |
| Analytical assay sensitivity | 0.6 pmol/l |
| Available format | 100 determinations |
Thyroid tumor marker
Immunoradiometric Assay for the determination Thyroglobulin (Tg) in human serum
| Assay principle | IRMA |
|---|---|
| Tracer | 125-Iod-anti-Tg + biotinyl. anti-Tg, monoclonal (mouse) |
| Standard range | 0,3 - 250 ng/ml |
| Reaction type | solid phase (coated tubes) |
| Total incubation time | over night |
| Sample volume | 100 µl |
| Functional assay sensitivity | 0,5 ng/ml |
| Available format | 100 determinations |
Immunoradiometric assay (coated tube) for the quantitative determination of human Calcitonin (hCT) in serum or plasma
| Assay principle | IRMA |
|---|---|
| Tracer | 125-Iod-anti-hCT mAb (mouse) |
| Standard range | 1 - 1800 pg/ml |
| Reaction type | solid phase (coated tube) |
| Total incubation time | 18 - 24 (5) hours |
| Sample volume | 100 µl |
| Functional assay sensitivity | 3 pg/ml |
| Available format | 100 determinations |
Type 1 Diabetes
Radio ligand assay for the quantitative determination of autoantibodies to Insulin
(IAA) in human serum
| Assay principle | Radio ligand assay |
|---|---|
| Tracer | 125-Iod-Insulin human |
| Standard range | 0.04 - 50 U/ml |
| Cut off | 0.4 U/ml |
| Reaction type | homogeneous phase |
| Total incubation time | over night + 60 min |
| Sample volume | 20 µl serum |
| Functional assay sensitivity | 0.2 U/ml |
| Available format | 100 determinations |
Radio ligand assay for the quantitative determination of autoantibodies to Protein Tyrosine Phosphatase IA2 (IA2 Ab) in human serum
| Assay principle | Radio ligand assay |
|---|---|
| Tracer | 125-Iod-IA2 human recombinant |
| Standard range | 0.01 - 60 U/ml |
| Cut off | 2.0 U/ml |
| Reaction type | homogeneous phase |
| Total incubation time | over night + 60 min |
| Sample volume | 20 µl serum |
| Functional assay sensitivity | 0.8 U/ml |
| Available format | 50 determinations |
Radio ligand assay for the quantitative determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Ab) in human serum
| Assay principle | Radio ligand assay |
|---|---|
| Tracer | 125-iod-GAD65 human recombinant |
| Standard range | 0.1 - 120 U/ml |
| Cut off | 2.0 U/ml |
| Reaction type | homogeneous phase |
| Total incubation time | 180 minutes |
| Sample volume | 20 µl |
| Functional assay sensitivity | 0.7 U/ml |
| Available format | 100 determinations |
Radio ligand assay for the quantitative determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Ab) in human serum
| Assay principle | Radio ligand assay |
|---|---|
| Tracer | 125-Iod-GAD65 human recombinant |
| Standard range | 0.1 - 120 U/ml |
| Cut off | 2.0 U/ml |
| Reaction type | homogeneous phase |
| Total incubation time | 180 minutes |
| Sample volume | 20 µl |
| Functional assay sensitivity | 0.7 U/ml |
| Available format | 50 determinations |
Radio ligand assay for the quantitative determination of autoantibodies to Protein Tyrosine Phosphatase IA2 (IA2 Ab) in human serum
| Assay principle | Radio ligand assay |
|---|---|
| Tracer | 125I-IA2 (human rec.) |
| Standard range | 0.01 - 60 U/ml |
| Cut off | 2.0 U/ml |
| Reaction type | homogeneous phase |
| Total incubation time | overnight + 60 min |
| Sample volume | 20 µl Serum |
| Functional assay sensitivity | 0.8 U/ml |
| Available format | 100 determinations |
