Thyroid autoantibodies

Radio receptor assay for the determination of TSH receptor autoantibodies (TRAb) in human serum - coated tubes technique (2. generation)

Assay principle Radio receptor assay (comp.)
Tracer 125-Jod-TSHR (mouse)
Standard range 1 - 50 IU/l (WHO 08/204)
Cut off 1.5 IU/l
Reaction type solid phase (coated tubes)
Total incubation time 180 minutes
Sample volume 100 µl
Functional assay sensitivity 1 IU/l
Available format 100 determinations

Radioimmunoassay for the determination of autoantibodies to Thyroid Peroxidase (TPO Ab) in human serum

Assay principle RIA (competitive)
Tracer 125-Iod-TPO (human)
Standard range 0 - 1900 IU/ml
Cut off 100 U/ml
Reaction type solid phase (coated tubes)
Total incubation time 120 minutes
Sample volume 10 µl
Functional assay sensitivity 12 IU/ml
Available format 100 determinations

Radioimmunoassay for the determination of autoantibodies to Thyroblobulin (Tg Ab) in neat serum. no predilution required

Assay principle RIA (competitive)
Tracer 125-iod-Tg (human)
Standard range 0 - 3000 IU/ml (NIBS 65/93)
Cut off 100 IU/ml
Reaction type solid phase (coated tubes)
Total incubation time 120 minutes
Sample volume 100 µl native serum
Functional assay sensitivity 12 IU/ml
Available format 100 determinations

Thyroid hormone

Immunoradiometric assay for the determination of Thyrotropin (TSH) in serum

Assay principle IRMA
Tracer 125-Iod-anti-hTSH mAb (mouse)
Standard range 0.06 - 100 mIU/l (IRP 80/558)
Reaction type solid phase (coated tubes)
Total incubation time 30 minutes
Sample volume 100 µl
Functional assay sensitivity 0.03 mIU/l
Available format 100 determinations

Radioimmuno assay for the determination of free Triiodothyronine (FT3) in human serum

Assay principle RIA (one step)
Tracer 125-iod-anti-T3 (monoclonal)
Standard range 0 - 40 pmol/l
Reaction type solid phase (coated tubes)
Total incubation time 120 minutes
Sample volume 100 µl serum
Available format 100 determinations

Radioimmuno assay for the determination of free Thyroxine (FT4) in human serum

Assay principle RIA (one step)
Tracer 125-Iod-anti-T4 (monoclonal)
Standard range 0 - 100 pmol/l
Reaction type solid phase (coated tubes)
Total incubation time 120 minutes
Sample volume 50 µl serum
Analytical assay sensitivity 0.7 pmol/l
Available format 100 determinations

Thyroid tumor marker

Immunoradiometric Assay for the determination Thyroglobulin (Tg) in human serum

Assay principle IRMA
Tracer 125-Iod-anti-Tg + biotinyl. anti-Tg, monoclonal (mouse)
Standard range 0,3 - 250 ng/ml
Reaction type solid phase (coated tubes)
Total incubation time over night
Sample volume 100 µl
Functional assay sensitivity 0,5 ng/ml
Available format 100 determinations

Immunoradiometric assay (coated tube) for the quantitative determination of human Calcitonin (hCT) in serum or plasma

Assay principle IRMA
Tracer 125-Iod-anti-hCT mAb (mouse)
Standard range 1 - 1800 pg/ml
Reaction type solid phase (coated tube)
Total incubation time 18 - 24 (5) hours
Sample volume 100 µl
Functional assay sensitivity 3 pg/ml
Available format 100 determinations

Type 1 Diabetes

Radio ligand assay for the quantitative determination of autoantibodies to Insulin

(IAA) in human serum

Assay principle Radio ligand assay
Tracer 125-Iod-Insulin human
Standard range 0.04 - 50 U/ml
Cut off 0.4 U/ml
Reaction type homogeneous phase
Total incubation time over night + 60 min
Sample volume 20 µl serum
Functional assay sensitivity 0.2 U/ml
Available format 100 determinations

Radio ligand assay for the quantitative determination of autoantibodies to Protein Tyrosine Phosphatase IA2 (IA2 Ab) in human serum

Assay principle Radio ligand assay
Tracer 125-Iod-IA2 human recombinant
Standard range 0.01 - 60 U/ml
Cut off 2.0 U/ml
Reaction type homogeneous phase
Total incubation time over night + 60 min
Sample volume 20 µl serum
Functional assay sensitivity 0.8 U/ml
Available format 50 determinations

Radio ligand assay for the quantitative determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Ab) in human serum

Assay principle Radio ligand assay
Tracer 125-iod-GAD65 human recombinant
Standard range 0.1 - 120 U/ml
Cut off 2.0 U/ml
Reaction type homogeneous phase
Total incubation time 180 minutes
Sample volume 20 µl
Functional assay sensitivity 0.7 U/ml
Available format 100 determinations

Radio ligand assay for the quantitative determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Ab) in human serum

Assay principle Radio ligand assay
Tracer 125-Iod-GAD65 human recombinant
Standard range 0.1 - 120 U/ml
Cut off 2.0 U/ml
Reaction type homogeneous phase
Total incubation time 180 minutes
Sample volume 20 µl
Functional assay sensitivity 0.7 U/ml
Available format 50 determinations