Indication Type 1 Diabetes

Radio ligand assay for the quantitative determination of autoantibodies to Insulin

(IAA) in human serum

Assay principle Radio ligand assay
Tracer 125-Iod-Insulin human
Standard range 0.04 - 50 U/ml
Cut off 0.4 U/ml
Reaction type homogeneous phase
Total incubation time over night + 60 min
Sample volume 20 µl serum
Functional assay sensitivity 0.2 U/ml
Available format 100 determinations

Radio ligand assay for the quantitative determination of autoantibodies to Protein Tyrosine Phosphatase IA2 (IA2 Ab) in human serum

Assay principle Radio ligand assay
Tracer 125-Iod-IA2 human recombinant
Standard range 0.01 - 60 U/ml
Cut off 2.0 U/ml
Reaction type homogeneous phase
Total incubation time over night + 60 min
Sample volume 20 µl serum
Functional assay sensitivity 0.8 U/ml
Available format 50 determinations

Radio ligand assay for the quantitative determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Ab) in human serum

Assay principle Radio ligand assay
Tracer 125-iod-GAD65 human recombinant
Standard range 0.1 - 120 U/ml
Cut off 2.0 U/ml
Reaction type homogeneous phase
Total incubation time 180 minutes
Sample volume 20 µl
Functional assay sensitivity 0.7 U/ml
Available format 100 determinations

Radio ligand assay for the quantitative determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Ab) in human serum

Assay principle Radio ligand assay
Tracer 125-Iod-GAD65 human recombinant
Standard range 0.1 - 120 U/ml
Cut off 2.0 U/ml
Reaction type homogeneous phase
Total incubation time 180 minutes
Sample volume 20 µl
Functional assay sensitivity 0.7 U/ml
Available format 50 determinations

Radio ligand assay for the quantitative determination of autoantibodies to Protein Tyrosine Phosphatase IA2 (IA2 Ab) in human serum

Assay principle Radio ligand assay
Tracer 125I-IA2 (human rec.)
Standard range 0.01 - 60 U/ml
Cut off 2.0 U/ml
Reaction type homogeneous phase
Total incubation time overnight + 60 min
Sample volume 20 µl Serum
Functional assay sensitivity 0.8 U/ml
Available format 100 determinations

Enzyme immunoassay for the determination of autoantibodies to Zinc Transporter 8 (ZnT8 Abs) in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with ZnT8
Conjugate ZnT8-Biotin, Streptavidin-POD
Substrate TMB
Standard range 1 – 500 U/ml
Cut off 15 U/ml
Total incubation time overnight + 100 minutes
Sample volume 25 µl serum
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Ab) in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with GAD65 (human recombinant)
Conjugate GAD65-Biotin, Streptavidin-POD
Substrate TMB
Standard range 5 - 250 IU/ml
Cut off 5 IU/ml
Total incubation time 160 minutes
Sample volume 25 µl serum
Functional assay sensitivity 4 IU/ml
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to Protein Tyrosine Phosphatase IA2 (IA2 Ab) in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with Protein Tyrosine Phosphatase (human recombinant)
Conjugate IA2-Biotin, Streptavidin-POD
Substrate TMB
Standard range 1 - 400 IU/ml
Cut off 10 IU/ml
Total incubation time over night + 100 minutes
Sample volume 50 µl serum
Functional assay sensitivity 0.8 IU/ml
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the semi-quantitative determination of autoantibodies to islet cell antigens in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with IC antigens
Conjugate anti-ICA-Biotin, Streptavidin-POD
Substrate TMB
Cut off Binding index = 1.0
Total incubation time overnight + 100 minutes
Sample volume 50 µl serum
Functional assay sensitivity BI < 0.5
Available format 96 determinations

Enzyme immunoassay for the determination of auto- (IAA) and antibodies (IA) to Insulin in human serum

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with human recombinant Insulin
Conjugate anti-human-gG-POD
Substrate TMB
Standard range 0.1 - 20 U/ml
Cut off 2.4 U/ml
Total incubation time 105 minutes
Sample volume 100 µl prediluted (1:100) serum (1+100, v+v)
Functional assay sensitivity 0.3 U/ml
Available format 96 determinations