Indication Thyroid Hormones

Immunoradiometric assay for the determination of Thyrotropin (TSH) in serum

Assay principle IRMA
Tracer 125-Iod-anti-hTSH mAb (mouse)
Standard range 0.06 - 100 mIU/l (IRP 80/558)
Reaction type solid phase (coated tubes)
Total incubation time 30 minutes
Sample volume 100 µl
Functional assay sensitivity 0.03 mIU/l
Available format 100 determinations

Radioimmuno assay for the determination of free Triiodothyronine (FT3) in human serum

Assay principle RIA (one step)
Tracer 125-iod-anti-T3 (monoclonal)
Standard range 0 - 40 pmol/l
Reaction type solid phase (coated tubes)
Total incubation time 60 minutes
Sample volume 100 µl serum
Analytical assay sensitivity 0.3 pmol
Available format 100 determinations

Radioimmuno assay for the determination of free Thyroxine (FT4) in human serum

Assay principle RIA (one step)
Tracer 125-Iod-anti-T4 (monoclonal)
Standard range 0 - 80 pmol/l
Reaction type solid phase (coated tubes)
Total incubation time 60 minutes
Sample volume 50 µl serum
Analytical assay sensitivity 0.6 pmol/l
Available format 100 determinations

Enzyme-linked immunosorbent assay for the determination of free Triiodothyronine (FT3) in serum

Assay principle Enzyme immunoassay (competitive)
Format microtiter plate coated with anti-T3 antibodies
Conjugate T3-POD
Substrate TMB
Standard range 0 - 60 pmol/l
Reference range 2.5 - 7.5 pmol/l
Total incubation time 70 minutes
Sample volume 20 µl serum
Analytical assay sensitivity 0.5 pmol/l
Available format 96 determinations

Enzyme-linked immunosorbent assay for the determination of free Thyroxine (FT4) in human serum

Assay principle Enzyme immunoassay (competitive, 1-step)
Format microtiter plate coated with anti-T4 antibodies
Conjugate T4-POD
Substrate TMB
Standard range 0.1 - 100 pmol/l
Reference range 10.0 - 23.2 pmol/l
Total incubation time 80 minutes
Sample volume 20 µl serum
Analytical assay sensitivity 1.0 pmol/l
Available format 96 determinations

Enzyme-linked immunosorbent assay for the high sensitive determination of Thyrotropin (TSH) in human serum (3. generation)

Assay principle Enzyme immunoassay (Sandwich, 1-step)
Format microtiter plate coated with anti-TSH antibodies
Conjugate anti-human-TSH-POD
Substrate TMB
Standard range 0.0 - 15 mlU/l
Reference range 0.3 - 3.5 mlU/l
Total incubation time 75 minutes
Sample volume 50 µl serum
Functional assay sensitivity 0.015 mlU/l
Available format 96 determinations