Enzymimmunoassays (EIA)

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to Thyroid Peroxidase (TPO Ab) in human serum

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with recombinant human TPO
Conjugate anti-human-IgG-POD
Substrate TMB
Standard range 50 - 3,000 IU/ml; (NIBSC 65/93)
Cut off 50 IU/ml
Total incubation time 105 min
Sample volume 100 µl diluted serum 1+100 (V+V)
Functional assay sensitivity 9.0 IU/ml
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to Thyroglobulin (Tg Ab) in human serum

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with highly purified human Tg
Conjugate anti -human-IgG-POD
Substrate TMB
Standard range 50 - 3,000 IU/ml (NIBSC 65/93)
Cut off 100 IU/ml
Total incubation time 105 minutes
Sample volume 100 µl diluted serum, 1 + 100 (V+V)
Functional assay sensitivity 9,0 IU/ml
Available format 96 determinations

Enzyme immunoassay (EIA) for the quantitative determination of autoantibodies to TSH receptor in human serum

Assay principle Enzyme immunoassay (competitive)
Format microtiter plate coated with TSH receptor (human)
Conjugate Anti-TSHR (mouse) - Biotin/Streptavidin-POD
Substrate TMB
Standard range 0.1 - 40 IU/l, (WHO 08/204)
Cut off 1.5 IU/l
Total incubation time 180 minutes
Sample volume 100 µl
Functional assay sensitivity 1.0 8 IU/l
Available format 96 determinations

Radioimmuno assays (RIA)

Radioreceptor assay for the determination of TSH receptor autoantibodies (TRAb) in human serum

Assay principle Radio receptor assay (comp.)
Tracer 125-Jod-anti-TSHR (mouse)
Standard range 1 - 50 IU/l (WHO 08/204)
Cut off 1.5 IU/l
Reaction type solid phase (coated tubes with human TSH receptor)
Total incubation time 180 minutes
Sample volume 100 µl native serum
Functional assay sensitivity 1 IU/l
Available format 100 determinations

Radioreceptor assay for the determination of TSH receptor autoantibodies (TRAb) in human serum

Assay principle Radio receptor assay (comp.)
Tracer 125-Jod-anti-TSHR (mouse)
Standard range 1 - 50 IU/l (WHO 08/204)
Cut off 1.5 IU/l
Reaction type solid phase (coated tubes with human TSH receptor)
Total incubation time 120 min
Sample volume 100 µl native serum
Functional assay sensitivity 0.9 IU/l
Available format 100 determinations

Radioimmunoassay for the determination of autoantibodies to Thyroid Peroxidase (TPO Ab) in human serum

Assay principle RIA (competitive)
Tracer 125-Iod-TPO (human)
Standard range 0 - 1900 IU/ml
Cut off 50 U/ml
Reaction type solid phase (coated tubes with polyclonal anti-TPO)
Total incubation time 120 minutes
Sample volume 10 µl
Functional assay sensitivity 12 IU/ml
Available format 100 determinations

Radioimmunoassay for the determination of autoantibodies to Thyroblobulin (Tg Ab) in neat serum. no predilution required

Assay principle RIA (competitive)
Tracer 125-iod-Tg (human)
Standard range 0 - 3000 IU/ml (NIBS 65/93)
Cut off 100 IU/ml
Reaction type solid phase (coated tubes)
Total incubation time 120 minutes
Sample volume 100 µl native serum
Functional assay sensitivity 12 IU/ml
Available format 100 determinations