AKLIDES® Tests - Cell based

Indirect immunofluorescence test for the determination of IgG antibodies

against neutrophil cytoplasmic antigens (ANCA) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with human granulocytes (ethanol fixed)
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 25 µl diluted serum
Available format 60 (10x6) determinations

Indirect immunofluorescence test for the determination of IgG antibodies

against nuclear (ANA) and cytoplasmic antigens in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with HEp-2 cells
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 25 µl diluted serum
Available format 480 (40x12) determinations

Indirect immunofluorescence test for the determination of IgG antibodies

against nuclear (ANA) and cytoplasmic antigens in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with HEp-2 cells
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 25 µl diluted serum
Available format 120 (10x12) determinations

Indirect immunofluorescence test for the determination of IgG antibodies

against neutrophil cytoplasmic antigens (ANCA) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with human granulocytes (formalin fixed)
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 25 µl diluted serum
Available format 60 (10x6) determinations

Indirect immunofluorescence test for the sensitive determination of IgG

antibodies against native DNA in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with Crithidia luciliae cells
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 25 µl diluted serum
Available format 60 (10x6) determinations

Indirect immunofluorescence test for the sensitive determination of IgG

antibodies against native DNA in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with Crithidia luciliae cells
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 25 µl diluted serum
Available format 120 (10x12) determinations

AKLIDES® Tests - Tissue based

Indirect immunofluorescence test for the determination of anti-mitochondrial antibodies (AMA, polyvalent) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with rat kidney sections
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of antibodies against smooth muscles (ASMA, polyvalent) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with rat stomach sections
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of antibodies (ANA/AMA/ASMA/PCA) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with sections of rat liver / stomach / kidney
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of antibodies (ANA/AMA/ASMA/PCA) in human serum

Assay principle Indirect immunofluorescence test
Format Slides with sections of rat liver / stomach / kidney
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 96 (12x8) determinations

Indirect immunofluorescence test for the determination of antibodies against the glomerular basement membrane (GBM) in human serum

Assay principle Indirect immunofluorescence test
Format Slides with monkey kidney sections
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of antibodies against skin antigens (ASA) in human serum

Assay principle Indirect immunofluorescence test
Format Slides with monkey esophagus
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of antibodies against islet cell antigens (ICA) in human serum

Assay principle Indirect immunofluorescence test
Format Slides with monkey pancreas
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of IgA antibodies against the endomysium (EmA) in human serum

Assay principle Indirect immunofluorescence test
Format Slides with monkey esophagus
Conjugate anti-human-IgA-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of IgA antibodies against the endomysium (EmA) in human serum

Assay principle Indirect immunofluorescence test
Format Slides with monkey esophagus
Conjugate anti-human-IgA-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted Serum
Available format 96 (12x8) determinations

Indirect immunofluorescence test for the determination of antibodies against cardiac muscle antigens (CMA) in human serum

Assay principle Indirect immunofluorescence test
Format Slides with monkey heart muscle
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of antibodies against skeletal muscle antigens (SkMA) in human serum

Assay principle Indirect immunofluorescence test
Format Slides with monkey skeletal muscle
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

Indirect immunofluorescence test for the determination of antibodies against adrenal cortex antigens in human serum

Assay principle Indirect immunofluorescence test
Format Slides with monkey adrenal cortex
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (12x4) determinations

AKLIDES® Tests - CytoBead

Indirect immunofluorescence assay for the determination of IgG antibodies against neutrophil cytoplasmic antigens (ANCA) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with human granulocytes (ethanol fixed) and beads coated with PR3, MPO and GBM
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (6x8) determinations

Indirect immunofluorescence assay for the determination of IgA or IgG antibodies against endomysium, transglutaminase 2 and deamidated gliadin as well as a positive control for IgA antibodies in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with monkey tissue sections (esophagus) and beads coated with TG2, DG and a-IgA
Conjugate anti-human-IgA-FITC / anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (6x8) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against nuclear and cytoplasmic antigens (ANA) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with HEp-2 cells and beads coated with La, CENP-B, Sm, RNP-Sm, dsDNA, Scl-70, Ro60 and Ro52
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 80 µl diluted serum
Available format 80 (10x8) determinations

Indirect immunofluorescence assay for the determination of IgG antibodies against nuclear and cytoplasmic antigens (ANA) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with HEp-2 cells and beads coated with La, Jo-1, Sm, RNP-Sm, dsDNA, Scl-70, Ro60 and Ro52
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 80 µl diluted serum
Available format 80 (10x8) determinations

Indirect immunofluorescence assay for the determination of IgG autoantibodies against antigens in cytoplasmic neutrophil granulocytes (ANCA), antibodies against antigenic structures of the glomerular basement membrane (GBM) and double stranded deoxyribonucleic acid (dsDNA) in human serum

Assay principle Indirect immunofluorescence assay
Format Slides with human granulocytes (ethanol fixed) and beads coated with PR3, MPO, GBM and dsDNA
Conjugate anti-human-IgG-FITC
Total incubation time 60 minutes
Sample volume 50 µl diluted serum
Available format 48 (6x8) determinations

AKLIDES® Tests - Cell Damage

Indirect immunofluorescence assay for the quantitative staining and determination of γ-H2AX in lymphocytes derived from whole blood

- FOR RESEARCH USE ONLY -

- Not for use in diagnostic procedures -

Assay principle Indirect immunofluorescence assay
Format Slides with 6 wells
Total incubation time 120 minutes
Sample volume 6 ml whole blood for preparation
Available format 30 determinations of duplicates (10x6)

Indirect immunofluorescence assay for the quantitative staining and determination of γ-H2AX and 53BP1 in lymphocytes derived from whole blood

- FOR RESEARCH USE ONLY -

- Not for use in diagnostic procedures -

Assay principle Indirect immunofluorescence assay
Format Slides with 6 wells
Total incubation time 120 minutes
Sample volume 6 ml whole blood for preparation
Available format 30 determinations of duplicates (10x6)

Lymphocyte isolation from whole blood

 

- FOR RESEARCH USE ONLY -

- Not for use in diagnostic procedures -

Assay principle Density gradient centrifugation
Format Slides with 6 wells
Sample volume 6 ml whole blood for preparation
Available format 30 determinations of duplicates (10x6)

Indirect immunofluorescence assay for the quantitative staining and determination of γ-H2AX and 53BP1 in lymphocytes from capillary blood

- FOR RESEARCH USE ONLY -

- Not for use in diagnostic procedures -