AKLIDES® Tests - Cell based
Indirect immunofluorescence test for the determination of IgG antibodies
against neutrophil cytoplasmic antigens (ANCA) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with human granulocytes (ethanol fixed) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 25 µl diluted serum |
| Available format | 60 (10x6) determinations |
Indirect immunofluorescence test for the determination of IgG antibodies
against nuclear (ANA) and cytoplasmic antigens in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with HEp-2 cells |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 25 µl diluted serum |
| Available format | 480 (40x12) determinations |
Indirect immunofluorescence test for the determination of IgG antibodies
against nuclear (ANA) and cytoplasmic antigens in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with HEp-2 cells |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 25 µl diluted serum |
| Available format | 120 (10x12) determinations |
Indirect immunofluorescence test for the determination of IgG antibodies
against neutrophil cytoplasmic antigens (ANCA) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with human granulocytes (formalin fixed) |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 25 µl diluted serum |
| Available format | 60 (10x6) determinations |
Indirect immunofluorescence test for the sensitive determination of IgG
antibodies against native DNA in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with Crithidia luciliae cells |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 25 µl diluted serum |
| Available format | 60 (10x6) determinations |
Indirect immunofluorescence test for the sensitive determination of IgG
antibodies against native DNA in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with Crithidia luciliae cells |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 25 µl diluted serum |
| Available format | 120 (10x12) determinations |
AKLIDES® Tests - Tissue based
Indirect immunofluorescence test for the determination of anti-mitochondrial antibodies (AMA, polyvalent) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with rat kidney sections |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of antibodies against smooth muscles (ASMA, polyvalent) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with rat stomach sections |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of antibodies (ANA/AMA/ASMA/PCA) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with sections of rat liver / stomach / kidney |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of antibodies (ANA/AMA/ASMA/PCA) in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with sections of rat liver / stomach / kidney |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 96 (12x8) determinations |
Indirect immunofluorescence test for the determination of antibodies against the glomerular basement membrane (GBM) in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with monkey kidney sections |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of antibodies against skin antigens (ASA) in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with monkey esophagus |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of antibodies against islet cell antigens (ICA) in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with monkey pancreas |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of IgA antibodies against the endomysium (EmA) in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with monkey esophagus |
| Conjugate | anti-human-IgA-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of IgA antibodies against the endomysium (EmA) in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with monkey esophagus |
| Conjugate | anti-human-IgA-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted Serum |
| Available format | 96 (12x8) determinations |
Indirect immunofluorescence test for the determination of antibodies against cardiac muscle antigens (CMA) in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with monkey heart muscle |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of antibodies against skeletal muscle antigens (SkMA) in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with monkey skeletal muscle |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
Indirect immunofluorescence test for the determination of antibodies against adrenal cortex antigens in human serum
| Assay principle | Indirect immunofluorescence test |
|---|---|
| Format | Slides with monkey adrenal cortex |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (12x4) determinations |
AKLIDES® Tests - CytoBead
Indirect immunofluorescence assay for the determination of IgG antibodies against neutrophil cytoplasmic antigens (ANCA) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with human granulocytes (ethanol fixed) and beads coated with PR3, MPO and GBM |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (6x8) determinations |
Indirect immunofluorescence assay for the determination of IgA or IgG antibodies against endomysium, transglutaminase 2 and deamidated gliadin as well as a positive control for IgA antibodies in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with monkey tissue sections (esophagus) and beads coated with TG2, DG and a-IgA |
| Conjugate | anti-human-IgA-FITC / anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (6x8) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against nuclear and cytoplasmic antigens (ANA) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with HEp-2 cells and beads coated with La, CENP-B, Sm, RNP-Sm, dsDNA, Scl-70, Ro60 and Ro52 |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 80 µl diluted serum |
| Available format | 80 (10x8) determinations |
Indirect immunofluorescence assay for the determination of IgG antibodies against nuclear and cytoplasmic antigens (ANA) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with HEp-2 cells and beads coated with La, Jo-1, Sm, RNP-Sm, dsDNA, Scl-70, Ro60 and Ro52 |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 80 µl diluted serum |
| Available format | 80 (10x8) determinations |
Indirect immunofluorescence assay for the determination of IgG autoantibodies against antigens in cytoplasmic neutrophil granulocytes (ANCA), antibodies against antigenic structures of the glomerular basement membrane (GBM) and double stranded deoxyribonucleic acid (dsDNA) in human serum
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with human granulocytes (ethanol fixed) and beads coated with PR3, MPO, GBM and dsDNA |
| Conjugate | anti-human-IgG-FITC |
| Total incubation time | 60 minutes |
| Sample volume | 50 µl diluted serum |
| Available format | 48 (6x8) determinations |
AKLIDES® Tests - Cell Damage
Indirect immunofluorescence assay for the quantitative staining and determination of γ-H2AX in lymphocytes derived from whole blood
- FOR RESEARCH USE ONLY -
- Not for use in diagnostic procedures -
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with 6 wells |
| Total incubation time | 120 minutes |
| Sample volume | 6 ml whole blood for preparation |
| Available format | 30 determinations of duplicates (10x6) |
Indirect immunofluorescence assay for the quantitative staining and determination of γ-H2AX and 53BP1 in lymphocytes derived from whole blood
- FOR RESEARCH USE ONLY -
- Not for use in diagnostic procedures -
| Assay principle | Indirect immunofluorescence assay |
|---|---|
| Format | Slides with 6 wells |
| Total incubation time | 120 minutes |
| Sample volume | 6 ml whole blood for preparation |
| Available format | 30 determinations of duplicates (10x6) |
Lymphocyte isolation from whole blood
- FOR RESEARCH USE ONLY -
- Not for use in diagnostic procedures -
| Assay principle | Density gradient centrifugation |
|---|---|
| Format | Slides with 6 wells |
| Sample volume | 6 ml whole blood for preparation |
| Available format | 30 determinations of duplicates (10x6) |
Indirect immunofluorescence assay for the quantitative staining and determination of γ-H2AX and 53BP1 in lymphocytes from capillary blood
- FOR RESEARCH USE ONLY -
- Not for use in diagnostic procedures -
