Thyroid autoantibodies

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to Thyroid Peroxidase (TPO Ab) in human serum

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with recombinant human TPO
Conjugate anti-human-IgG-POD
Substrate TMB
Standard range 50 - 3,000 IU/ml; (NIBSC 65/93)
Cut off 50 IU/ml
Total incubation time 105 min
Sample volume 100 µl diluted serum 1+100 (V+V)
Functional assay sensitivity 9.0 IU/ml
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to Thyroglobulin (Tg Ab) in human serum

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with highly purified human Tg
Conjugate anti -human-IgG-POD
Substrate TMB
Standard range 50 - 3,000 IU/ml (NIBSC 65/93)
Cut off 100 IU/ml
Total incubation time 105 minutes
Sample volume 100 µl diluted serum, 1 + 100 (V+V)
Functional assay sensitivity 9,0 IU/ml
Available format 96 determinations

Enzyme immunoassay (EIA) for the quantitative determination of autoantibodies to TSH receptor in human serum

Assay principle Enzyme immunoassay (competitive)
Format microtiter plate coated with TSH receptor (human)
Conjugate Anti-TSHR (mouse) - Biotin/Streptavidin-POD
Substrate TMB
Standard range 0.1 - 40 IU/l, (WHO 08/204)
Cut off 1.5 IU/l
Total incubation time 180 minutes
Sample volume 100 µl
Functional assay sensitivity 1.0 8 IU/l
Available format 96 determinations

Thyroid tumor marker

Enzyme-linked Immunosorbent Assay for quantitative determination of Thyroglobulin (Tg) in human serum

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with antibody to thyroglobulin (mouse, monoclonal)
Conjugate anti-hTg-POD
Substrate TMB
Standard range 0 - 200 ng/ml (CRM 457)
Total incubation time 75 minutes
Sample volume 50 µl Serum
Functional assay sensitivity 1.9 ng/ml
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of Thyroglobulin in human serum

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with antibodies to thyroglobulin (mouse, monoclonal)
Conjugate anti-hTg-POD
Substrate TMB
Standard range 0 - 3 ng/ml (CRM 457)
Total incubation time overnight + 165 min
Sample volume 25 µl serum
Functional assay sensitivity 0.03 ng/ml
Available format 96 determinations

Thyroid hormone

Enzyme-linked immunosorbent assay for the determination of free Triiodothyronine (FT3) in serum

Assay principle Enzyme immunoassay (competitive)
Format microtiter plate coated with anti-T3 antibodies
Conjugate T3-POD
Substrate TMB
Standard range 0 - 60 pmol/l
Reference range 2.5 - 7.5 pmol/l
Total incubation time 70 minutes
Sample volume 20 µl serum
Analytical assay sensitivity 0.5 pmol/l
Available format 96 determinations

Enzyme-linked immunosorbent assay for the determination of free Thyroxine (FT4) in human serum

Assay principle Enzyme immunoassay (competitive, 1-step)
Format microtiter plate coated with anti-T4 antibodies
Conjugate T4-POD
Substrate TMB
Standard range 0.1 - 100 pmol/l
Reference range 10.0 - 23.2 pmol/l
Total incubation time 80 minutes
Sample volume 20 µl serum
Analytical assay sensitivity 1.0 pmol/l
Available format 96 determinations

Enzyme-linked immunosorbent assay for the high sensitive determination of Thyrotropin (TSH) in human serum (3. generation)

Assay principle Enzyme immunoassay (Sandwich, 1-step)
Format microtiter plate coated with anti-TSH antibodies
Conjugate anti-human-TSH-POD
Substrate TMB
Standard range 0.0 - 15 mlU/l
Reference range 0.3 - 3.5 mlU/l
Total incubation time 75 minutes
Sample volume 50 µl serum
Functional assay sensitivity 0.015 mlU/l
Available format 96 determinations

Typ 1 Diabetes

Enzyme immunoassay for the determination of autoantibodies to Zinc Transporter 8 (ZnT8 Abs) in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with ZnT8
Conjugate ZnT8-Biotin, Streptavidin-POD
Substrate TMB
Standard range 1 – 500 U/ml
Cut off 15 U/ml
Total incubation time overnight + 100 minutes
Sample volume 25 µl serum
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Ab) in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with GAD65 (human recombinant)
Conjugate GAD65-Biotin, Streptavidin-POD
Substrate TMB
Standard range 5 - 250 IU/ml
Cut off 5 IU/ml
Total incubation time 160 minutes
Sample volume 25 µl serum
Functional assay sensitivity 4 IU/ml
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to Protein Tyrosine Phosphatase IA2 (IA2 Ab) in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with Protein Tyrosine Phosphatase (human recombinant)
Conjugate IA2-Biotin, Streptavidin-POD
Substrate TMB
Standard range 1 - 400 IU/ml
Cut off 10 IU/ml
Total incubation time over night + 100 minutes
Sample volume 50 µl serum
Functional assay sensitivity 0.8 IU/ml
Available format 96 determinations

Enzyme-linked immunosorbent assay (ELISA) for the semi-quantitative determination of autoantibodies to islet cell antigens in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with IC antigens
Conjugate anti-ICA-Biotin, Streptavidin-POD
Substrate TMB
Cut off Binding index = 1.0
Total incubation time overnight + 100 minutes
Sample volume 50 µl serum
Functional assay sensitivity BI < 0.5
Available format 96 determinations

Enzyme immunoassay for the determination of auto- (IAA) and antibodies (IA) to Insulin in human serum

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with human recombinant Insulin
Conjugate anti-human-gG-POD
Substrate TMB
Standard range 0.1 - 20 U/ml
Cut off 2.4 U/ml
Total incubation time 105 minutes
Sample volume 100 µl prediluted (1:100) serum (1+100, v+v)
Functional assay sensitivity 0.3 U/ml
Available format 96 determinations

Rheumatoid Arthritis

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to cyclic citrullinated peptides in human serum or plasma

Assay principle Enzyme immunoassay (Sandwich)
Format microtiter plate coated with CCP antigens
Conjugate anti-human IgG POD
Substrate TMB
Standard range 1 - 2,000 U/ml
Cut off 30 U/ml
Total incubation time 105 minutes
Sample volume 100 µl serum diluted serum (1+100, V+V)
Functional assay sensitivity 2 U/ml
Available format 96 determinations

Neuropathies

Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of autoantibodies to acetylcholine receptor in human serum

Assay principle Enzyme immunoassay (competitive)
Format microtiter plate coated with AChR, antibodies, pre-absorption with AChR preparation
Conjugate anti-AChR-Biotin, Streptavidin-POD
Substrate TMB
Standard range 0.2 - 20 U/ml
Cut off 0.45 nmol/l
Total incubation time overnight + 180 minutes
Sample volume 100 µl prediluted serum (1+100, V+V)
Available format 96 determinations

Polyendocrinopathies

Enzyme immunoassay for the quantitative determination of autoantibodies to 21-Hydroxylase (21-OH Ab) in human serum

Assay principle Enzyme immunoassay (Bridge)
Format microtiter plate coated with 21-OH (human recombinant)
Conjugate 21-OH-Biotin, Streptavidin-POD
Substrate TMB
Standard range 0.01 - 100 U/ml
Cut off 0.4 U/ml
Total incubation time overnight + 100 min
Sample volume 50 µl serum
Analytical assay sensitivity 0.13 U/ml
Available format 96 determinations