AKLIDES® CytoBead®

The automated way of CytoBead®
The automated way of CytoBead®

The AKLIDES® CytoBead® ANCA, ANA, ANA 2, CeliAK and RPGN test kits are used for the diagnosis of systemic and organ specific autoimmune disease, via indirect immunofluorescence.

The AKLIDES® CytoBead® ANCA, ANA, ANA 2, CeliAK and RPGN test kits consist of reagents to determine IgG autoantibodies against neutrophil cytoplasmic antigens (CytoBead® ANCA and CytoBead® RPGN), nuclear and cytoplasmic antigens (CytoBead® ANA and CytoBead® ANA 2) and IgA or IgG antibodies against endomysium, transglutaminase 2 and deamidated gliadin (CytoBead® CeliAK).

  • The never before seen CytoBead® assays combine sensitive screening using native substrates (tissue sections or cells) as well as specific diagnostic confirmation using solid phase assays (microparticles), in one reaction environment
  • Using the familiar method of indirect immunofluorescence, the processing of AKLIDES® CytoBead® assays is made fast and reliable
  • The use of standard glass slides guarantees simple handling
  • The evaluation of CytoBead® assays  can be done eithervmanually or automatically, using the AKLIDES® system
  • With automatic evaluation using the AKLIDES® system, quantitative results comparable to ELISA are produced.

All CytoBead® assays are based on the principle of indirect immunofluorescence. With the aid of a native substrate (tissue sections or cells), as well as a multiplex-ready solid phase (beads), IgG and IgA autoantibodies from human serum can be detected in one reaction environment. In the first step, autoantibodies present in the diluted human serum react with the substrates on the cells and tissues, as well as with the corresponding autoantigens on the fluorescence coded beads.

In the second step, the specific detection of the antigen-antibody reaction takes place using fluorophore labelled secondary antibodies.

Assay principle

Indirect immunofluorescence

Assay size

48 (6x8) determinations for AKLIDES® CytoBead® ANCA, CeliAK and RPGN as well as 80 (10x8) determinations for AKLIDES® CytoBead® ANA or CytoBead® ANA2

Parameter

Human granulocytes (ethanol fixated) and microparticles coated with PR3, MPO and GBM (AKLIDES® CytoBead® ANCA)

Human granulocytes (ethanol fixated) and microparticles coated with PR3, MPO, GBM and dsDNA (AKLIDES® CytoBead® RPGN)

HEp-2 cells and microparticles coated with  La, CENP-B, Sm, RNP-Sm, dsDNA, Scl-70, Ro60 and Ro52 (AKLIDES® CytoBead® ANA)

HEp-2 cells and microparticles coated with  La, Jo-1, Sm, RNP-Sm, dsDNA, Scl-70, Ro60 and Ro52 (AKLIDES® CytoBead® ANA 2)

Monkey tissue sections (esophagus) and microparticles coated with tTG, DG and a-IgA (AKLIDES® CytoBead® CeliAK)

Result

Detection of IgG autoantibodies / IgA autoantibodies (AKLIDES® CytoBead® CeliAK) in human serum

Incubation time

60 Minutes

Sample material

50 - 80 µl diluted serum

The CytoBead® principle combines the traditional stepwise diagnostic approach of screening and confirmatory testing, into one test run. This saves time during processing, reduces material costs, and means less patient sample is used up.

Grossmann, K., Röber, N., Hiemann, R., Rödiger, S., Schierack, P., Reinhold, D., Laass, M.-W., Conrad, K. & Roggenbuck, D. Simultaneous detection of celiac disease-specific IgA antibodies and total IgA. Auto Immun Highlights. 2016 Dec; 7(1): 2.

 

Scholz, J., Grossmann, K., Knütter, I., Hiemann, R., Sowa,  M., Röber, N., Rödiger, S., Schierack, P., Reinhold, D., Bogdanos, D.P., Meroni, P.L., Radice, A., Conrad, K. & Roggenbuck, D. Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing. Clin Chem Lab Med 2015; 53(12): 1991-2002.

 

Sowa, M., Grossmann, K., Knütter, I., Hiemann, R., Röber, N., Anderer, U., Csernok, E., Bogdanos, D.P., Borghi, M.O., Meroni, P.L., Schierack, P., Reinhold, D., Conrad, K. & Roggenbuck, D. Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA. PLoS One. 2014; 9(9): e107743.

 

Sowa, M., Grossmann, K., Scholz, J., Röber, N., Rödiger, S., Schierack, P., Conrad, K., Roggenbuck, D. & Hiemann, R. The CytoBead assay - a novel approach of multiparametric autoantibody analysis in the diagnostics of systemic autoimmune diseases. J Med Lab 2014; 38: 309-17.