AKLIDES® Cell Damage

AKLIDES® 2.0 Nuk

Biomarkers for the determination of DNA double strand breaks, the so-called DNA repair foci, can be standardized and automatically measured and evaluated using the AKLIDES® cell damage system. The quantitative determination of DNA repair foci via immunofluorescence is a very sensitive method, which is able to evaluate both single cells and single foci. Compared to manual analysis, such as visual counting of DNA repair foci in a dark room, the AKLIDES® cell damage system offers the following advantages:

  • Standardized and objective evaluation of DNA repair foci
  • Faster analysis compared to visual counting
  • Automatic determination of DNA repair foci using various fluorescent dyes
  • Detailed results through the measurement of various parameters, including average number of foci per cell, percentage of cells with foci, mean intensity of foci, average diameter of foci and cells
  • Archiving of results and images.

The AKLIDES® Cell Damage system consists of a fluorescence microscope, grayscale camera, sample stage holding up to five slides, LED light source with four wavelengths, as well as a PC including the AKLIDES® Cell Damage software. The software controls both the hardware components and the automatic image processing and analysis. Focusing on nuclei happens in the DAPI channel, and then their fluorescence signals are measured and analyzed in the corresponding signals, depending on the fluorescence dye used. The software is able to record multiple z-levels, as well as analyzing clustered foci, which cannot be analyzed manually.

The use of non-standardized protocols for sample preparation, and the subsequent manual evaluation of DNA repair foci, means that test results from different studies are not comparable. Using automatic analysis with the AKLIDES® Cell Damage system removes the subjective factor of visual counting, and so for the first time it is possible to compare test results from studies worldwide. 

With the AKLIDES® Cell Damage system, at least 30 patient samples per day can be measured and analyzed. With the manual counting on DNA double strand breaks, using a fluorescence microscope in a darkroom, this number is much lower. In addition, the subjective factor of this strenuous and time consuming manual procedure is eliminated.

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